3TSV
crystal structure of the third PDZ domain of the human ZO-1 MAGUK protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.497, 45.117, 59.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.980 - 1.989 |
| R-factor | 0.27326 |
| Rwork | 0.269 |
| R-free | 0.31526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lh5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.980 | 2.110 |
| High resolution limit [Å] | 1.989 | 1.989 |
| Rmerge | 0.050 | 0.610 |
| Number of reflections | 6649 | |
| <I/σ(I)> | 15.1 | 2.1 |
| Completeness [%] | 97.8 | 93.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 25% PEG 1500, 0.1M MMT, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
