3TSR

X-ray structure of mouse ribonuclease inhibitor complexed with mouse ribonuclease 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	72.396, 125.341, 123.064
Unit cell angles	90.00, 94.72, 90.00

Refinement procedure

Resolution	34.378 - 2.200
R-factor	0.1827
Rwork	0.182
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.2.4)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.145	0.071	0.478
Number of reflections	108499
<I/σ(I)>	6.1
Completeness [%]	97.9	99.4	88.2
Redundancy	4.2	4.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	298	Protein solution mixed in a 1:1 ratio with the well solution (PEG 3350 25%, NaCitrate 100mM pH 3.5), vapor diffusion, hanging drop. cryoprotected with 20% Ethylene Glycol, PEG 3350 25%, and NaCitrate 100mM pH 3.5, temperature 298K