3TS0
Mouse Lin28A in complex with let-7f-1 microRNA pre-element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 139.880, 139.880, 85.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.980 - 2.763 |
| R-factor | 0.1941 |
| Rwork | 0.193 |
| R-free | 0.21450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3trz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.980 | 2.772 |
| High resolution limit [Å] | 2.763 | 2.763 |
| Rmerge | 0.060 | 0.629 |
| Number of reflections | 22328 | |
| <I/σ(I)> | 28.2 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 0.6M NaH2PO4, 1.4M K2HPO4, pH 7, vapor diffusion, temperature 298K |
