3TR5
Structure of a peptide chain release factor 3 (prfC) from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.254, 141.429, 161.869 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 41.965 - 2.110 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.761 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.098 | 0.060 | 0.620 |
| Number of reflections | 143670 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 98.3 | 96.3 | 90.8 |
| Redundancy | 3.6 | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.1M MES pH 6.0, 0.2M calcium acetate, 20% PEG 8000 , VAPOR DIFFUSION, SITTING DROP, temperature 295K |
