3TQ7
EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-07-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.150, 103.560, 48.130 |
| Unit cell angles | 90.00, 113.64, 90.00 |
Refinement procedure
| Resolution | 44.090 - 2.300 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wu9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.095 | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 4.000 | 2.300 |
| Number of reflections | 15694 | ||
| <I/σ(I)> | 27.64 | 57.88 | 5.42 |
| Completeness [%] | 95.2 | 99.2 | 99.1 |
| Redundancy | 13.4 | 13 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 15% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
