3TOW

Crystal Structure of the MABP Domain of MVB12B of Human ESCRT-I Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	32.371, 52.607, 71.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.537 - 1.340
R-factor	0.1663
Rwork	0.165
R-free	0.19310
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.465
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.540	1.360
High resolution limit [Å]	1.340	1.340
Rmerge	0.079	0.520
Number of reflections	27812
<I/σ(I)>	9.9	2.017
Completeness [%]	99.5	93.3
Redundancy	7.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	33% PEG 400, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	33% PEG 400, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K