3TOC

Crystal structure of Streptococcus pyogenes Csn2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-02
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97910, 0.97927, 0.96404
Spacegroup name	C 2 2 21
Unit cell lengths	59.482, 163.362, 149.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.173 - 2.201
R-factor	0.2175
Rwork	0.215
R-free	0.25840
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.087
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	36996
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.3M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K