3TO4
Structure of mouse Valpha14Vbeta2-mouseCD1d-alpha-Galactosylceramide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95453 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.211, 82.594, 238.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.050 - 3.100 |
| R-factor | 0.21965 |
| Rwork | 0.217 |
| R-free | 0.26753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3he7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.019 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.640 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.241 | 1.243 |
| Number of reflections | 22054 | |
| <I/σ(I)> | 10.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 17% PEG 10K, 0.1M ammonium acetate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
