3TNU

Heterocomplex of coil 2B domains of human intermediate filament proteins, keratin 5 (KRT5) and keratin 14 (KRT14)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Wavelength(s)	0.97944
Spacegroup name	H 3 2
Unit cell lengths	150.991, 150.991, 141.623
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.528 - 3.005
R-factor	0.2103
Rwork	0.209
R-free	0.23350
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.821
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.005
Number of reflections	12376
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.2	K5/K14 2B protein solution (9 mg/mL) was mixed with an equal volume of reservoir solution containing 2.8M NaCl and 0.1M Tris-HCl (pH 8.5). Small crystals formed and were used to seed the same mixture of protein solution/reservoir solution, which yielded larger crystals, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K