3TNF

LidA from Legionella in complex with active Rab8a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.97860
Spacegroup name	P 43 21 2
Unit cell lengths	103.820, 103.820, 150.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.2189
Rwork	0.216
R-free	0.27470
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.600
High resolution limit [Å]	2.500	15.000	2.500
Rmerge	0.052	0.030	0.593
Number of reflections	29149
<I/σ(I)>	26.34	56.21	4.59
Completeness [%]	99.8	99.3	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	50% (v/v) MPD , 0.1 M sodium cacodylate pH 5.6, 10 mM spermidine, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	50% (v/v) MPD , 0.1 M sodium cacodylate pH 5.6, 10 mM spermidine, vapor diffusion, hanging drop, temperature 293K