3TMM

TFAM imposes a U-turn on mitochondrial DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97864, 0.91837, 0.97898
Spacegroup name	C 2 2 21
Unit cell lengths	68.438, 81.911, 161.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.192 - 2.500
R-factor	0.1973
Rwork	0.195
R-free	0.24780
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.192	25.000	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge	0.074
Number of reflections	15812
<I/σ(I)>	10.6
Completeness [%]	99.8	87.9	99.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	29% PEG 400, 0.15 M calcium acetate, 0.1 M sodium acetate,pH 4.2, 400 mM NDSB211, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	29% PEG 400, 0.15 M calcium acetate, 0.1 M sodium acetate,pH 4.2, 400 mM NDSB211, VAPOR DIFFUSION, HANGING DROP, temperature 298K