3TLZ
Microcin C7 self immunity protein MccF mutant W186F in complex with Adenosine Monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2010-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.553, 90.189, 73.322 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.17405 |
| Rwork | 0.173 |
| R-free | 0.19272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tla |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.800 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.067 | 0.261 |
| Number of reflections | 115009 | |
| <I/σ(I)> | 38.094 | 5.593 |
| Completeness [%] | 97.8 | 95 |
| Redundancy | 5.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 282 | 0.1M HEPES pH 7.5, 10% PEG 8000, 8% Ethylene Glycol, vapor diffusion, temperature 282K, VAPOR DIFFUSION |
