3TLR

Crystal Structure of the tetrameric Beta-2 microglobulin DIMC20 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-07-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.97633
Spacegroup name	P 21 21 21
Unit cell lengths	29.930, 98.200, 142.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.450
R-factor	0.2279
Rwork	0.225
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x4s
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	BUSTER (2.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.580
High resolution limit [Å]	2.450	2.450
Number of reflections	15721
Completeness [%]	97.2	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	293	Hepes 0.1 M pH 7.7, CdSO4 0.12 M, sodium acetate 2.4 M, VAPOR DIFFUSION, SITTING DROP, temperature 293K