3TLG

Microcin C7 self immunity protein MccF in the inactive mutant APO state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	54.507, 85.808, 73.198
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	26.133 - 1.499
R-factor	0.1628
Rwork	0.161
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.068
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.499	3.230	1.499
Rmerge	0.069	0.059	0.121
Number of reflections	205542
<I/σ(I)>	20.5
Completeness [%]	99.7	98.1	99.8
Redundancy	4.6	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		282	vapor diffusion, temperature 282K