3TL4
Crystal Structure of the tRNA Binding Domain of Glutaminyl-tRNA Synthetase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97904, 0.91162, 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.800, 34.620, 74.269 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 26.299 - 2.300 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.034 |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.615 | 26.299 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.033 | 0.490 | |
| Total number of observations | 1866 | 9002 | |
| Number of reflections | 9393 | ||
| <I/σ(I)> | 13 | 19.2 | 1.6 |
| Completeness [%] | 99.9 | 98.3 | 100 |
| Redundancy | 6.5 | 5.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 295 | 100mM KCl, 100mM Tris, 20% (w/v) PEG 4000, pH 8.0, microbatch under oil, temperature 295K |
