3TJO

HtrA1 catalytic domain, mutationally inactivated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Collection date	2010-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	.98
Spacegroup name	P 43 21 2
Unit cell lengths	151.922, 151.922, 87.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.301
R-factor	0.18566
Rwork	0.185
R-free	0.21616
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	wild-type homologue
RMSD bond length	0.009
RMSD bond angle	1.191
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	44800
<I/σ(I)>	19
Completeness [%]	98.0
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	ammonium sulfate, LiCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K