3TID
Crystal structure of the LCMV derived peptide GP34 in complex with the murine mhc class I H-2 Kb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-10-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.089 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.261, 47.649, 70.539 |
Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
Resolution | 39.376 - 1.650 |
R-factor | 0.1762 |
Rwork | 0.175 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.164 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.000 | 45.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Number of reflections | 64779 | ||
Completeness [%] | 97.0 | 98.3 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 8K, Cacodylate, Magnesium Acetate, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |