3THV
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddATP-dT in Closed Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97121 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.810, 109.270, 150.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.408 - 1.611 |
R-factor | 0.1947 |
Rwork | 0.193 |
R-free | 0.22440 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hvi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.266 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.710 |
High resolution limit [Å] | 1.610 | 4.830 | 1.610 |
Rmerge | 0.066 | 0.033 | 0.493 |
Number of reflections | 193977 | ||
<I/σ(I)> | 14.23 | 37.26 | 3.58 |
Completeness [%] | 97.2 | 98.7 | 91.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 45-50% saturated ammonium sulfate, 2.5% MPD, 10 mM magnesium sulfate, 100 mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |