3THF

Crystal structure of the SD2 domain from Drosophila Shroom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-04
Detector	ADSC QUANTUM
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	72.797, 85.632, 92.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.633 - 2.695
R-factor	0.2317
Rwork	0.228
R-free	0.28380
Structure solution method	SAD
RMSD bond length	0.100
RMSD bond angle	0.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.695	7.320	2.695
Rmerge	0.069	0.044	0.463
Number of reflections	16446
<I/σ(I)>	9.7
Completeness [%]	99.9	99.5	99.9
Redundancy	8.5	7.7	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	K/Na tartrate, sodium thiocynate, glycerol, pH 6.0, vapor diffusion, temperature 277K
1	VAPOR DIFFUSION	6	277	K/Na tartrate, sodium thiocynate, glycerol, pH 6.0, vapor diffusion, temperature 277K