3THC
Crystal structure of human beta-galactosidase in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.799, 116.104, 140.326 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 28.880 - 1.800 |
| R-factor | 0.19217 |
| Rwork | 0.190 |
| R-free | 0.22833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d3a |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.430 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.880 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 250825 |
| Completeness [%] | 78.0 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (w/v) PEG3350 0.2M ammonium sulfate, 100mM Tris HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
