3TGZ
Crystal Structure Analysis of W35F/H207W Mutant of Human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.947, 47.639, 68.097 |
| Unit cell angles | 90.00, 106.44, 90.00 |
Refinement procedure
| Resolution | 58.290 - 2.300 |
| R-factor | 0.2436 |
| Rwork | 0.240 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o3t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.605 |
| Data reduction software | SAINT (V7.68A) |
| Data scaling software | SAINT (V7.68A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.290 | 1.952 | |
| High resolution limit [Å] | 1.884 | 4.059 | 1.884 |
| <I/σ(I)> | 75.242 | ||
| Completeness [%] | 98.0 | ||
| Redundancy | 8.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 293 | 0.1 M Bis-Tris, 20% (v/v) PEG 3350 monomethyl ether, 0.2 M sodium acetate, 2.5 mM DTT, pH 6.5, hanging drop, temperature 293K |
