3TG6
Crystal Structure of Influenza A Virus nucleoprotein with Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 146.692, 146.692, 146.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.240 - 3.000 |
| R-factor | 0.23528 |
| Rwork | 0.233 |
| R-free | 0.29633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.850 | 3.180 |
| High resolution limit [Å] | 2.820 | 2.950 |
| Number of reflections | 21313 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.25 | 293 | 10 mM NaAc pH 4.5, 0.1 M NaCl, 0.1 M NaCitrate pH 5.25, 0.5 M LiCl, 16% PEG 4000, VAPOR DIFFUSION, temperature 293K |
