3TG6
Crystal Structure of Influenza A Virus nucleoprotein with Ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 146.692, 146.692, 146.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.240 - 3.000 |
R-factor | 0.23528 |
Rwork | 0.233 |
R-free | 0.29633 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.850 | 3.180 |
High resolution limit [Å] | 2.820 | 2.950 |
Number of reflections | 21313 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.25 | 293 | 10 mM NaAc pH 4.5, 0.1 M NaCl, 0.1 M NaCitrate pH 5.25, 0.5 M LiCl, 16% PEG 4000, VAPOR DIFFUSION, temperature 293K |