3TG1
Crystal structure of p38alpha in complex with a MAPK docking partner
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.428, 72.428, 226.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.214 - 2.710 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1P38 2ouc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.214 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.078 | 0.359 |
| Number of reflections | 17026 | |
| <I/σ(I)> | 20.6 | 2.58 |
| Completeness [%] | 98.5 | 93.3 |
| Redundancy | 6.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 100mM Tris pH7.5, 9% [w/v] polyethylene glycol 3350, 8% [w/v] sucrose, VAPOR DIFFUSION, temperature 293K |
