3TFM
Myosin X PH1N-PH2-PH1C tandem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 36.840, 36.840, 154.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.530 |
| R-factor | 0.22117 |
| Rwork | 0.215 |
| R-free | 0.27775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fao |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.066 | 0.333 |
| Number of reflections | 5972 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 96.2 | 96.7 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 20%(w/v) PEG8000, 0.05M potassium phosphate monobasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
