3TD1

Crystal structure of the bacterial A1408G-mutant and the protozoa cytoplasmic ribosomal decoding site in complex with geneticin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	33.470, 88.300, 46.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.900 - 2.100
Rwork	0.196
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mwl
RMSD bond length	0.005
RMSD bond angle	0.800
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.900	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.065	0.359
Number of reflections	8461
Completeness [%]	99.9	100
Redundancy	6.9	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Cacodylate, Spermine tetrahydrochloride, Ammonium chloride, 2-methyl-2,4-pentanediol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K