3TCQ
Crystal Structure of matrix protein VP40 from Ebola virus Sudan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-30 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.982, 90.940, 48.684 |
Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
Resolution | 27.368 - 1.600 |
R-factor | 0.19582 |
Rwork | 0.194 |
R-free | 0.22052 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dut |
RMSD bond length | 0.010 |
RMSD bond angle | 1.590 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.368 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.066 | 0.482 |
Number of reflections | 36225 | |
<I/σ(I)> | 10.821 | 2.481 |
Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 291 | 11mg/ml EbsuA.17247.a PD00181, 10% PEG4000, 20% glycerol, 0.1M MES pH 6.5. Direct cryoprotection. , VAPOR DIFFUSION, SITTING DROP, temperature 291K |