3TCK

Crystal structure of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis - Form 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-07-03
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	36.300, 38.810, 122.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.224
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z2i
RMSD bond length	0.008
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.158
Number of reflections	7780
<I/σ(I)>	11.4
Completeness [%]	95.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch under oil	7.5	291	0.1M HEPES, 15% PEG 8000, 5% isoproponal, pH 7.5, Microbatch under oil, temperature 291K