3TC7
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR62.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97915 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.960, 60.960, 118.663 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.440 - 1.500 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1igs |
RMSD bond length | 0.006 |
RMSD bond angle | 1.092 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.073 | 0.491 |
Number of reflections | 41081 | |
<I/σ(I)> | 29.3 | 3.2 |
Completeness [%] | 98.4 | 92.5 |
Redundancy | 7.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 5 | 277 | 40 % PEG 400, 0.1 M Ammonium phosphate monobasic, 0.1 M Sodium acetate, pH 5.0, Microbatch crystallization under oil, temperature 277K |