3TC3
Crystal Structure of SacUVDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.075, 53.585, 77.395 |
| Unit cell angles | 102.10, 93.02, 111.77 |
Refinement procedure
| Resolution | 46.050 - 1.500 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6v |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.211 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.050 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.099 | 0.520 |
| Number of reflections | 94666 | |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 97.3 | 94 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 20 % PEG3350, 0.25 M NH4Cl, pH 7.0, VAPOR DIFFUSION, temperature 298K |
