3TBI
Crystal structure of T4 gp33 bound to E. coli RNAP beta-flap domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X3A |
Synchrotron site | NSLS |
Beamline | X3A |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9785 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 55.218, 112.250, 164.847 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.995 - 3.000 |
R-factor | 0.25293 |
Rwork | 0.251 |
R-free | 0.29485 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.656 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.995 |
High resolution limit [Å] | 3.000 |
Number of reflections | 10551 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 295 | 0.1 M triethylamine N-oxide, 20% PEG2000 MME, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, temperature 295K |