3TBB
Small laccase from Streptomyces viridosporus T7A; alternate crystal form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-20 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 127.000, 127.000, 155.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.931 - 2.300 |
R-factor | 0.1632 |
Rwork | 0.161 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tas |
RMSD bond length | 0.008 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_764) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.930 | 2.300 |
Rmerge | 0.214 | 0.017 | 0.017 |
Number of reflections | 64657 | 6835 | 6219 |
<I/σ(I)> | 15.12 | 42.78 | 2.23 |
Completeness [%] | 99.9 | 99.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 282 | Protein concentrationw as 20 mg/ml in Tris-HCl (pH 7.9); the precipitant contained 5% 2-propanol, and 2.5 M dibasic potassium phosphate/monobasic sodium phosphate (pH 5.5)., VAPOR DIFFUSION, HANGING DROP, temperature 282K |