3TAM
Crystal structure of HIV-1 reverse transcriptase (K103N mutant) in complex with inhibitor M06
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.910, 153.900, 155.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.580 - 2.510 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3lp1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BUSTER (2.9.4) |
| Refinement software | BUSTER (2.9.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.510 |
| Rmerge | 0.080 |
| Number of reflections | 42589 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 87.4 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 298 | sodium citrate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
