3T9J

Crystal structure HP-NAP from strain YS39 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-03-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	F 4 3 2
Unit cell lengths	189.465, 189.465, 189.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.340 - 2.200
R-factor	0.22465
Rwork	0.223
R-free	0.24812
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ji4
RMSD bond length	0.006
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.064	0.365
Number of reflections	15374
<I/σ(I)>	66.4	11.2
Completeness [%]	99.9	100
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	0.1M L-arginine, 20% ethylene glycol, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K