3T85
Human Carbonic Anhydrase II in complex with Acetylated Carbohydrate Sulfamates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.94723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.466, 41.419, 72.156 |
| Unit cell angles | 90.00, 104.73, 90.00 |
Refinement procedure
| Resolution | 24.676 - 2.400 |
| R-factor | 0.1618 |
| Rwork | 0.158 |
| R-free | 0.23240 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18336 | |
| Completeness [%] | 99.6 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 289 | 2.9M (NH4)2SO4, 0.1M Glycine, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
