3T7O
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese, UDP-Glucose and glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-12-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.415, 46.590, 69.904 |
| Unit cell angles | 79.54, 88.75, 75.83 |
Refinement procedure
| Resolution | 28.330 - 1.850 |
| R-factor | 0.19809 |
| Rwork | 0.195 |
| R-free | 0.25501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.094 | 0.424 |
| Number of reflections | 44222 | |
| <I/σ(I)> | 9.5 | 2.8 |
| Completeness [%] | 92.9 | 88.5 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 2.0M Ammonium Sulphate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
