3T72

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	200
Detector technology	CCD
Collection date	2006-06-27
Detector	MAR CCD 165 mm
Wavelength(s)	1.2542,1.2554,1.2498
Spacegroup name	C 1 2 1
Unit cell lengths	277.300, 161.400, 260.100
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	50.000 - 4.330
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.330
Number of reflections	73615
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	8-10% PEG 4000, 100 mM KCl, 10 mM magnesium chloride, 50 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K