3T6Q

Crystal structure of mouse RP105/MD-1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	143.753, 192.790, 75.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.020 - 1.900
R-factor	0.18805
Rwork	0.186
R-free	0.23128
RMSD bond length	0.013
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	115.240
High resolution limit [Å]	1.900
Number of reflections	155204
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	19% (w/v) PEG 8000, 100mM Tris HCl (pH 9.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K