3T5W
2ME modified human SOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.086, 163.277, 173.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.040 - 1.800 |
| R-factor | 0.20181 |
| Rwork | 0.200 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p1v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.700 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 185279 | |
| <I/σ(I)> | 12 | 1.6 |
| Completeness [%] | 94.9 | 88.2 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 15% PEG 3350, 300mM (NH4)2SO4, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
