3T5T

Vall from streptomyces hygroscopicus in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Collection date	2011-03-21
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	80.671, 46.693, 123.879
Unit cell angles	90.00, 108.08, 90.00

Refinement procedure

Resolution	40.390 - 1.700
R-factor	0.18061
Rwork	0.179
R-free	0.20921
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t7d
RMSD bond length	0.009
RMSD bond angle	1.221
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.965
High resolution limit [Å]	1.700
Number of reflections	90507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP