3T53

Crystal structures of the extrusion state of the CusBA adaptor-transporter complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	H 3 2
Unit cell lengths	159.787, 159.787, 683.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.120 - 3.370
R-factor	0.2496
Rwork	0.248
R-free	0.28580
Structure solution method	MR-SAD
RMSD bond length	0.003
RMSD bond angle	0.619
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.490
High resolution limit [Å]	3.370	7.250	3.370
Rmerge	0.148	0.037
Number of reflections	42148
<I/σ(I)>	5.2
Completeness [%]	99.6	98.7	99.9
Redundancy	2.9	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	10% PEG3350, 0.4M NH4SO4,10% Glycerol, pH 7.0, vapor diffusion, temperature 298K