3T4K
Arabidopsis histidine kinase 4 sensor domain in complex with N-benzyladenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.140, 60.140, 297.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.110 - 1.770 |
| R-factor | 0.18154 |
| Rwork | 0.180 |
| R-free | 0.20963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t4j |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.386 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.110 | 1.820 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 62050 | |
| <I/σ(I)> | 17.02 | 2.8 |
| Completeness [%] | 99.4 | 91.6 |
| Redundancy | 10.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 16% PEG 3,350, 0.2 M Na malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
