3T3T
1.38 A structure of human frataxin variant Q148G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0971 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.410, 68.560, 89.970 |
| Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
| Resolution | 26.190 - 1.380 |
| R-factor | 0.19947 |
| Rwork | 0.198 |
| R-free | 0.22656 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.500 |
| Data reduction software | XFIT |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.190 | 1.416 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 111516 | |
| Completeness [%] | 96.7 | 94.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 2.0 M ammonium sulfate, 0.1 M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
