3T3N
Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9800 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 101.950, 116.780, 231.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.410 - 3.090 |
| R-factor | 0.2066 |
| Rwork | 0.202 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bk1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS (Package) |
| Data scaling software | XDS (Package) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 3.250 |
| High resolution limit [Å] | 3.090 | 3.100 |
| Rmerge | 0.100 | 0.641 |
| <I/σ(I)> | 11.67 | 1.93 |
| Completeness [%] | 94.6 | 85.1 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2-4% PEG 4K, 0.02M ammonium sulfate, 0.1M Na-MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
