3T3F
Ternary Structure of the large fragment of Taq DNA polymerase bound to an abasic site and dNITP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.778, 109.778, 91.219 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.155 - 1.900 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ktq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.7.1_74) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.935 | |
| Number of reflections | 50510 | |
| <I/σ(I)> | 1.58 | |
| Completeness [%] | 99.6 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M TRIS HCl, 0.2 M MgFormate, 18% PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
