3T1X
MglB R124A E127A Monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54180 |
| Spacegroup name | I 4 |
| Unit cell lengths | 74.250, 74.250, 76.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.050 - 2.910 |
| R-factor | 0.40864 |
| Rwork | 0.405 |
| R-free | 0.46861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t1s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.050 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 4223 |
| Completeness [%] | 89.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293.15 | 0.1M CHES, 30% PEG 400 , pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
