3T1W
Structure of the four-domain fragment Fn7B89 of oncofetal fibronectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.980, 29.115, 82.241 |
| Unit cell angles | 90.00, 111.42, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.23008 |
| Rwork | 0.228 |
| R-free | 0.27693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18943 | |
| <I/σ(I)> | 18.8 | 3.5 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 26 %(w/v) PEG3350, 0.1 M Tris-HCl, 0.2 M NaCl, 5 %(v/v) Isopropanol, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
