3T1S
MglB Homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9300 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 36.150, 36.150, 308.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.300 - 1.670 |
| R-factor | 0.2212 |
| Rwork | 0.219 |
| R-free | 0.27229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j3w |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.300 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 15213 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | 0.28M CaCl2, 7% Isopropanol, 30% Glycerol, 0.07 M Natrium-Acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
