3T15
Structure of green-type Rubisco activase from tobacco
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0332 |
Spacegroup name | P 65 |
Unit cell lengths | 103.635, 103.635, 56.675 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.950 |
R-factor | 0.2292 |
Rwork | 0.226 |
R-free | 0.29620 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.449 |
Data reduction software | XDS |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 51.848 | 56.725 | 3.110 |
High resolution limit [Å] | 2.949 | 9.330 | 2.950 |
Rmerge | 0.062 | 0.061 | 0.505 |
Total number of observations | 1564 | 3960 | |
Number of reflections | 7453 | ||
<I/σ(I)> | 14.6 | 8.6 | 1.5 |
Completeness [%] | 99.9 | 98.6 | 100 |
Redundancy | 4 | 6.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 50 mM MES-Na pH 6.0 and 350 mM magnesium formate, vapor diffusion, temperature 291K |