3T15
Structure of green-type Rubisco activase from tobacco
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 65 |
| Unit cell lengths | 103.635, 103.635, 56.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.2292 |
| Rwork | 0.226 |
| R-free | 0.29620 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.848 | 56.725 | 3.110 |
| High resolution limit [Å] | 2.949 | 9.330 | 2.950 |
| Rmerge | 0.062 | 0.061 | 0.505 |
| Total number of observations | 1564 | 3960 | |
| Number of reflections | 7453 | ||
| <I/σ(I)> | 14.6 | 8.6 | 1.5 |
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 4 | 6.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 50 mM MES-Na pH 6.0 and 350 mM magnesium formate, vapor diffusion, temperature 291K |
