3T0Q

Motor Domain Structure of the Kar3-like kinesin from Ashbya gossypii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	44.866, 84.623, 101.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.640 - 2.350
R-factor	0.212
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kar
RMSD bond length	0.008
RMSD bond angle	1.283
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.077	0.244
Number of reflections	16073
<I/σ(I)>	16.1
Completeness [%]	95.6	97.8
Redundancy	3.8	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	293	14% PEG 3350, 125 mM sodium malonate, 1 mM TCEP and 0.1 M Bis-Tris propane pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K