3SZ5

Crystal Structure of LHK-Exo in complex with 5-phosphorylated oligothymidine (dT)4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-01-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	108.697, 108.697, 48.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.1958
Rwork	0.193
R-free	0.24772
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3syy
RMSD bond length	0.014
RMSD bond angle	1.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.492
Number of reflections	8198
<I/σ(I)>	35.5	3.4
Completeness [%]	99.9	100
Redundancy	6.8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	0.1M Tris pH 8.4, 1.0M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K